MfN Data Repository

doi:10.7479/dft0-yy6m
author(s):Willsch, M.
date of publication:2019
abstract: This dataset is the data supplement of:
Willsch M, Friedrich F, Baum D, Jurisch I, Ohl M (2020). A comparative description of the mesosomal musculature in Sphecidae and Ampulicidae (Hymenoptera, Apoi ...
This dataset is the data supplement of:
Willsch M, Friedrich F, Baum D, Jurisch I, Ohl M (2020). A comparative description of the mesosomal musculature in Sphecidae and Ampulicidae (Hymenoptera, Apoidea) using 3D techniques. Deutsche Entomologische Zeitschrift. https://doi.org/10.3897/dez.67.49493


Using microCT and 3D techniques, detailed insights of the skeletal musculature of apoid wasp species are illustrated. Focus was set on the mesothorax, metathorax and propodeum of Sceliphron destillatorium, Sphex (Fernaldina) lucae (both Sphecidae), and Ampulex compressa (Ampulicidae). MicroCT scanning was accomplished in the Visualisation Laboratory of the Museum für Naturkunde Berlin using a Phoenix nanotom X-ray|s tube (General Electric) at 48–50kV and 250–275µA. At 1 second per image 1000–1440 projections were generated per scan. The different kV- and projection-settings depended on the respective specimen size, which was also responsible for the range of the effective voxel size between 3.4–5μm. The cone beam reconstruction was performed using the CT reconstruction software PHOENIX|X-RAY DATOS|X 2.0 (GE Sensing & Inspection Technologies GmbH). The reconstructed images were processed using the software AMIRA ZIB EDITION 2020.02 and former versions. The muscles were segmented and labelled manually by using appropriate segmentation tools in AMIRA. Segmented materials were transformed into high-resolution surfaces using the Isosurface-Tool in AMIRA. The reconstruction was accomplished for one body side of the specimens. Post-editing was made by exporting TIF-files from AMIRA into PHOTOSHOP CS6. Besides these figures, all raw data are provided for reproducibility and further research.
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license: CC BY-SA

Raw µCT Data

Figure 1 - Portraits of the three species examined


  • Figure 1A. - Sceliphron destillatorium, lateral view, body size 20mm.


  • Figure 1B. - Sceliphron destillatorium, dorsal view, body size 20mm.


  • Figure 1C. - Sphex (Fernaldina) lucae, lateral view, body size 18mm.


  • Figure 1D. - Sphex (Fernaldina) lucae, dorsal view, body size 18mm.


  • Figure 1E. - Ampulex compressa, lateral view, body size 21mm.


  • Figure 1F. - Ampulex compressa, dorsal view, body size 21mm.

Figure 2 - Volume rendering of the mesosomal exoskeleton of the three species examined

Abbreviations: N1l – pronotal lobe, cx1 – procoxa, cx2 – mesocoxa, cx3 – metacoxa, tr2 – mesotrochanter, S1 – prosternum, S2 – mesosternum, S3 – metasternum.

  • Figure 2A. - Sceliphron, dorsal surface view, head left. Scale bar 0.9mm.


  • Figure 2B. - Sceliphron, lateral surface view, head left. Scale bar 0.9mm.


  • Figure 2C. - Sceliphron, ventral surface view, head left. Scale bar 1mm.


  • Figure 2D. - Sphex, dorsal surface view, head left. Scale bar 0.9mm.


  • Figure 2E. - Sphex, lateral surface view, head left. Scale bar 1mm.


  • Figure 2F. - Sphex, ventral surface view, head left. Scale bar 1mm.


  • Figure 2G. - Ampulex, dorsal surface view, head left. Scale bar 1mm.


  • Figure 2H. - Ampulex, lateral surface view, head left. Scale bar 1mm.


  • Figure 2I. - Ampulex, ventral surface view, head left. Scale bar 1mm.

Figure 3 - Sceliphron destillatorium, volume rendering, mesosomal musculature

Abbreviations: fu2-fu1v – ventral mesofurco-profurcal; pl2-t2a – first mesopleuro-mesonotal; pl2-ba2 – mesopleuro-mesobasalar; sp1occ – anterior thoracic spiracle occlusor; ism1,2-ba2 – intersegmental membrane-mesobasalar; pl2-3ax2a – first mesopleuro-third axillary sclerite of fore wing; pl2-3ax2b – second mesopleuro-third axillary sclerite of fore wing; pl2-3ax2c – third mesopleuro-third axillary sclerite of fore wing; pl2-t2b – second mesopleuro-mesonotal; cx2-sa2 – mesocoxo-mesosubalar; fu2a-ph2 – anterior mesofurco-mesolaterophragmal; pl2a-fu2 – anterior mesopleuro-mesofurcal; pl2-cx2 – mesopleuro-mesocoxal; s2-cx2 – mesosterno-mesocoxal; fu2-cx2 – mesofurco-mesocoxal; fu2l-tr2 – lateral mesofurco-mesotrochanteral; fu2m-tr2 – median mesofurco-mesotrochanteral; ph1-ph2 – prophragmo-mesophragmal; pl3a-ba3 – anterior metapleuro-metabasalar; t2p-t3 – posterior mesonoto-metanotal; pl3la-t3 – anterolateral metapleuro-metanotal; pl3d-3ax3 – dorsal metapleuro-third axillary sclerite of hind wing; pl3-sa3 – metapleuro-metasubalar; cx3-sa3 – metacoxo-metasubalar; pl3m-cx3 – median metapleuro-metacoxal; fu3l-cx3 – lateral metafurco-metacoxal; fu3m-cx3 – median metafurco-metacoxal; pl3l-cx3 – lateral metapleuro-metacoxal; fu3-tr3 – metafurco-metatrochanteral; pl3-tr3 – metapleuro-metatrochanteral; ph2m-ph3 – median mesophragmo-metaphragmal; ph3-T2 – metaphragmo-second abdominal tergal; T1-S2 – propodeo-second abdominal sternal; fu3-S2 – metafurco-second abdominal sternal; s3-S2 – metasterno-second abdominal sternal.

Scale bars A–C = 0.8mm, D = 0.9mm

  • Figure 3A. - Medial view, anterior to the right; muscles discernible from the centre.


  • Figure 3B. - Medial view, anterior to the right; muscles positioned sublateral.


  • Figure 3C. - Medial view, anterior to the right; muscles located sublateral and lateral.


  • Figure 3D. - Lateral view, anterior to the left; laterally positioned muscles.

Figure 4 - The mesosomal musculature of Sphex (Fernaldina) lucae divergent to S. destillatorium; volume rendering, transparent exoskeleton

Abbreviations: sp3occ – posterior thoracic spiracle occlusor; sp2 – posterior spiracle; pl2-cx2 – mesopleuro-mesocoxal; pl2-cx2b – second mesopleuro-mesocoxal; fu2l-tr2 – lateral mesofurco-mesotrochanteral; fu2m-tr2 – median mesofurco-mesotrochanteral; s3-cx3 – metasterno-metacoxal; pl3m-cx3 – median metapleuro metacoxal; fu3l-cx3 – lateral metafurco metacoxal; fu3m-cx3 – median metafurco metacoxal; pl3l-cx3 – lateral metapleuro metacoxal; mepr – mesepimeral ridge.

Scale bar A = 0.7mm, B = 0.6mm, C = 0.3mm.

  • Figure 4A. - Lateral view, anterior to the left.


  • Figure 4B. - Medial view, anterior to the right.


  • Figure 4C. - Anterior view on the posterior thoracic spiracle occlusor.

Figure 5 - The mesosomal musculature of Ampulex compressa divergent to Sphecidae; volume rendering, transparent exoskeleton

Abbreviations: sp1occ – anterior thoracic spiracle occlusor; ism1,2-ba2 – intersegmental membrane-mesobasalar; pl2-t2b – second mesopleuro-mesonotal; sp3occ – posterior thoracic spiracle occlusor; s2-cx2 – mesosterno-mesocoxal; pl2-cx2 – mesopleuro-mesocoxal; pl2-cx2b – second mesopleuro-mesocoxal; fu2-cx2 – mesofurco-mesocoxal; fu2m-tr2 – median mesofurco-mesotrochanteral; pl3a-ba3 – anterior metapleuro-metabasalar; t2p-t3 – posterior mesonoto-metanotal; pl3la-t3 – anterolateral metapleuro-metanotal; pl3lp-t3 – posterolateral metapleuro-metanotal; pl3v-3ax3 – ventral metapleuro-third axillary sclerite of hind wing; pl3-sa3 – metapleuro-metasubalar; pc3l-fu3 – lateral metapecto-metafurcal; fu3-cx3 – metafurco-metacoxal; fu3l-cx3 – lateral metafurco-metacoxal; fu3m-cx3 – median metafurco-metacoxal; pl3l-cx3 – lateral metapleuro-metacoxal; fu3-tr3 – metafurco-metatrochanteral; pl3-tr3 – metapleuro-metatrochanteral; ph3-T2 – metaphragmo-second abdominal tergal; T1-S2 – propodeo-second abdominal sternal; fu3-S2 – metafurco-second abdominal sternal; s3-S2 – metasterno-second abdominal sternal.

Scale bars A–C = 0.7mm, D and E = 0.8mm, F–H = 0.6mm.

  • Figure 5A. - Medial view, anterior to the right, all relevant muscles visible from the centre.


  • Figure 5B. - Medial view, anterior to the right, submedial muscles.


  • Figure 5C. - Medial view, anterior to the right, further lateral located muscles.


  • Figure 5D. - Lateral view, anterior to the left, all relevant muscles located lateral.


  • Figure 5E. - Lateral view, anterior to the left, muscles located sublateral.


  • Figure 5F. - Lateral view, anterior to the left, muscles located further medial.


  • Figure 5G. - Lateral view, anterior to the left, all newly identified muscles (plus pl2-cx2).


  • Figure 5H. - Dorsolateral view, anterior to the left, all newly identified muscles (plus pl2-cx2).

Figure 6 - Comparison of the paracoxal ridge (pcr) and metapleural apodeme (pl3a)

Further abbreviations: pl3lp-t3 – posterolateral metapleuro-metanotal muscle, pl3-tr3 – metapleuro-metatrochanteral muscle, fu3-tr3 – metafurcor-metatrochanteral muscle.

Scale bars A–C = 0.4mm, D = 0.3mm.

  • Figure 6A. - Sceliphron destillatorium, anterolateral view, head left.


  • Figure 6B. - Sphex lucae, anterolateral view, head left.


  • Figure 6C. - Weakly developed structures in Ampulex compressa, lateral view, head left.


  • Figure 6D. - Ampulex compressa, paracoxal ridge, lateral metafurcal arms (fu3a) fused with reduced metapleural apodeme, anterior view.

Figure 7 - Comparison of fu2m-tr2 – median mesofurco-mesotrochanteral muscle and fu2l-tr2 – lateral mesofurco-mesotrochanteral muscle, anterolateral view

Scale bars A = 0.4mm, B = 0.5mm.

  • Figure 7A. - Sceliphron destillatorium.


  • Figure 7B. - Ampulex compressa.

Figure 8 - Comparison of t2p-t3 – posterior mesonoto-metanotal muscle, posteromedial view

Scale bars = 0.2mm.

  • Figure 8A. - Sceliphron destillatorium


  • Figure 8B. Ampulex compressa.

Figure 9 - Comparison of pl3-sa3 – metapleuro-metasubalar muscle, dorsolateral view, anterior to the left

Further abbreviations: mpa – mesopleural apodeme; mtpa – metapleural apodeme; pl3 – metapleuron; sa3 – metasubalare; sp2 – posterior thoracic spiracle.

Scale bars A = 0.2mm, B = 0.4mm.

  • Figure 9A. - Sceliphron destillatorium


  • Figure 9B. - Ampulex compressa.

Figure 10 - Illustration of the metaphragma (ph3) in the propodeum (pr) of Ampulex compressa


  • Figure 10A. - Medial view on ph3-T2 – metaphragmo-second abdominal tergal muscle, anterior to the right.


  • Figure 10B. - Anteromedial view on ph3-T2 – metaphragmo-second abdominal tergal muscle.


  • Figure 10C. - Anteromedial view on the metaphragma (ph3).


  • Figure 10D. - Posterior view on the vertical part of propodeum (pr).